Invitrogen™ pHrodo™ Red Phagocytosis Particle Labeling Kit for Flow Cytometry

Invitrogen™ pHrodo™ Red Phagocytosis Particle Labeling Kit for Flow Cytometry

The pHrodo™ Phagocytosis Particle Labeling Kit for flow cytometry allows rapid labeling of biological particles, such as bacteria and assessment of phagocytic activity in whole blood samples by flow cytometry.

Features

• The pHrodo™ Red dye gives faster and more accurate results than any other phagocytosis assay, The pHrodo™ Red dye gives faster and more accurate results than any other phagocytosis assa The new Molecular Probes™ proprietary pH-sensitive rhodamine-based pHrodo™ Red dye is non-fluorescent at neutral pH, but turns bright red upon acidification. Because it is both fluorogenic and pH-sensitive, the pHrodo™ Red dye can be used as a specific sensor of phagocytic events; acidification of the phagosome following phagocytosis is marked by red fluorescence. It is therefore an ideal tool with which to study phagocytosis and its regulation by drugs and/or environmental factors.
• Rapid and reproducible, Wash steps and quencher dyes are not needed since the pHrodo™ Red dye is non-fluorescent outside the cell, making the staining protocol simple and fast. The elimination of wash steps and quencher dyes also improves assay reproducibility, particularly in plate reader-based assays.
• Flexible applications, Use ready-made pHrodo™ Red BioParticles™ conjugates for convenient analysis of phagocytosis of Gram-positive or Gram-negative bacteria, or the amine-reactive pHrodo™ Red SE form for labeling microorganisms or proteins of your choice.
• Compatible with multiple platforms, pHrodo™ Red dye conjugates can be used in plate readers, fluorescence microscopy imaging, and flow cytometry applications. The pHrodo™ Red Phagocytosis Particle Labeling Kit and the pHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for flow cytometry were designed for rapid and convenient measurements of phagocytic activity in whole blood samples by flow cytometry. The kits include all the reagents required for assessing particle ingestion and red blood cell lysis. The Labeling Kit also contains the reagents required for labeling microorganisms with the pHrodo™ Red dye. Sufficient reagents are provided in both kits for approximately 100 assays.

Contents

• Lysis buffer (10 mL).
• Buffer B (200 mL).
• Wash buffer (200 mL).
• 1 Vial pHrodo™ red succinimidyl ester (1 mg, lyophilized product).
• 1 Vial DMSO (0.5 mL).
• 1 Bottle sodium bicarbonate (50 mL, 0.1 M, pH 9.3).