Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry APC

Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry APC

The eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

Reactivity/Species, Bovine, Canine, Cow, Human, Monkey, Mouse, Non-human primate, Rat, Rhesus.

Conjugate, APC.

Excite, 633 - 647 nm.

Contents

• BrdU: 5 x 1 mL sterile vials, store at less than or equal to -70°C.
• DNase I: 10 x 0.3 mL vials, store at less than or equal to -70°C.
• Anti-BrdU APC Antibody (clone BU20A): 1 x 100 test vial, store at 2-8°C.
• BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle, store at 2-8°C.
• Fixation/Permeabilization Diluent: 1 x 100 mL bottle, store at 2-8°C.