Invitrogen™ pTrcHis TOPO™ TA Expression Kit
On demandInvitrogen™ pTrcHis TOPO™ TA Expression Kit
The pTrcHis- and pTrcHis2-TOPO® TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO® or pTrcHis2-TOPO®, includes a minicistron element for enhanced translation efficiency of eukaryotic proteins in E. coli. Both vectors are provided linearized and activated with topoisomerase I for 5-minute TOPO® Cloning with >85% cloning efficiency. To simplify protein purification and detectio.
pTrcHis-TOPO® Features
- N-terminal Xpress™ epitope for detection with an Anti-Xpress™ Antibody
- N-terminal polyhistidine (6xHis) tag for purification using nickelchelating resin and detection with an Anti-HisG Antibody
- Enterokinase cleavage site for efficient removal of N-terminal fusion tags
pTrcHis2-TOPO® Features
C-terminal c-myc epitope for detection with an Anti-myc Antibody
C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
Contents
- The TOPO™ TA box contains 200 ng of linearized
- topoisomerase I-activated pTrcHis-TOPO™ vector
- pTrcHis2-TOPO™ vector
- sterile water
- dNTPs
- 10X PCR buffer
- a control template and primers
- primers for sequencing or PCR screening
- an expression control plasmid
- The One Shot™ TOP10 box contains twenty-one
- 50-µL aliquots of chemically competent TOP10 E. coli
- S.O.C. medium
- a supercoiled control plasmid.
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