Invitrogen™ pTrcHis TOPO™ TA Expression Kit

Invitrogen™ pTrcHis TOPO™ TA Expression Kit

The pTrcHis- and pTrcHis2-TOPO® TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO® or pTrcHis2-TOPO®, includes a minicistron element for enhanced translation efficiency of eukaryotic proteins in E. coli. Both vectors are provided linearized and activated with topoisomerase I for 5-minute TOPO® Cloning with >85% cloning efficiency. To simplify protein purification and detectio.

pTrcHis-TOPO® Features

• N-terminal Xpress™ epitope for detection with an Anti-Xpress™ Antibody
• N-terminal polyhistidine (6xHis) tag for purification using nickelchelating resin and detection with an Anti-HisG Antibody
• Enterokinase cleavage site for efficient removal of N-terminal fusion tags

pTrcHis2-TOPO® Features

C-terminal c-myc epitope for detection with an Anti-myc Antibody

C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody

Contents

• The TOPO™ TA box contains 200 ng of linearized
• topoisomerase I-activated pTrcHis-TOPO™ vector
• pTrcHis2-TOPO™ vector
• sterile water
• dNTPs
• 10X PCR buffer
• a control template and primers
• primers for sequencing or PCR screening
• an expression control plasmid
• The One Shot™ TOP10 box contains twenty-one
• 50-µL aliquots of chemically competent TOP10 E. coli
• S.O.C. medium
•  a supercoiled control plasmid.