Invitrogen™ GeneArt™ Type IIs Assembly Kit, Aar I

Invitrogen™ GeneArt™ Type IIs Assembly Kit, Aar I

The GeneArt® Type IIs Assembly Kit, Aar I, provides seamless cloning and assembly of up to 8 DNA fragments by simultaneous cleavage and ligation in a single reaction. The kit uses a technology similar to Golden Gate cloning with the type IIs restriction enzyme Aar I and can be used to assemble multiple fragments in a pre-determined order into any compatible vector. Since type IIs assembly is not based upon homologous recombination, there is minimal risk of rearrangements and minimal sequence confirmation of your final construct is required.

Features

•  Assemble multiple DNA fragments in any order, into any compatible vector, without scars
• Avoid homologous recombination and associated rearrangements when cloning homologous or repetitive sequences
• Use for assembly of TALs, gene variants, and repetitive sequences
• Create your own cloning and expression vectors with custom vector elements
• Minimize sequence confirmation of final construct
• Pick from three type IIs enzymes