Invitrogen™ Baculovirus Expression System with Gateway™ Technology

Invitrogen™ Baculovirus Expression System with Gateway™ Technology

The Baculovirus Expression System with Gateway® Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. 

Features

• Expression using the Bac-to-Bac® technology (1)
• Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
• pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)

Contents

• The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer
• destination vectors pDEST™8
• pDEST™10
• pDEST™20
• proteinase K solution (2 µg/µl)
• pENTR™-gus positive control
• Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells.