Invitrogen™ Qubit™ RNA BR Assay Kit, 100 Reactions

Invitrogen™ Qubit™ RNA BR Assay Kit, 100 Reactions

The Qubit RNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng/µL to 1 µg/µL providing an assay range from 20–1,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. 

For use with equipment: Qubit™ Fluorometer.

Features

• Core range (high confidence): 0.1 µg/mL to 5 µg/mL
• Extended ranges (moderate confidence): 0.05 µg/mL to 0.1 µg/mL and 5 µg/mL to 6 µg/mL
• Useful for quantitation of samples for microarray, RT-PCR, and northern blot procedures
• Accurate in the presence of DNA, salts, solvents, proteins, and free nucleotides
• Excitation/Emission: 644/673
• For Use With (Equipment): Qubit Fluorometer
• Quantitation Range: 20 to 1000 ng
• Shipping Condition: Room Temperature
• Detection Method: Fluorescent 

Contents

• Qubit® RNA BR Reagent (Component A) , 250 μL 
• Qubit® RNA BR Buffer (Component B), 50 mL 
• Qubit® RNA BR Standard #1 (Component C), 1 mL
• Qubit® RNA BR Standard #2 (Component D), 4 × 250 μL