Invitrogen Rat IgM Isotype Control (eBRM), PE-eFluor™ 610, 25 µg, eBioscience™
On demandInvitrogen Rat IgM Isotype Control (eBRM), PE-eFluor™ 610, 25 µg, eBioscience™
The monoclonal rat IgM, kappa is useful as an isotype control immunoglobulin.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits
at 607 nm. We recommend using a 610/20 band pass filter (equivalent to
PE-Texas Red®). Please make sure that your instrument is capable of
detecting this fluorochome.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL of
cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact
on brightness and FRET efficiency/compensation. Some generalizations
regarding fluorophore performance after fixation can be made, but clone
specific performance should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.
There are no specifications
There are no report
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