Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix

The SuperScript™ III First-Strand Synthesis SuperMix is an optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.1 pg to 5 µg of total RNA.

Contents

• 2X Reaction Mix (500 µl)
• SuperScript™ III Enzyme Mix (100 µl)
• Oligo(dT)20 (50 µl total provided at 50 µM)
• Random hexamers (50 µl total provided at 50 ng/µl)
• Annealing buffer (50 µl)