Invitrogen™ MitoProbe™ DiOC2(3) Assay Kit, For Flow Cytometry, 100 Assays

Invitrogen™ MitoProbe™ DiOC2(3) Assay Kit, For Flow Cytometry, 100 Assays

The MitoProbe™ DiOC₂(3) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiOC₂(3), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiOC₂(3) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, red fluorescence. DiOC₂(3) staining intensity decreases with decreased mitochondrial membrane potential, accompanied by a shift from red to green fluorescence.

Contents

• 1 vial of DiOC₂(3) (625 μL, 10 μM in DMSO).
• 1 vial of CCCP: (125 μL, 50 mM DMSO).