Invitrogen™ Vybrant™ DyeCycle™ Ruby Stain, 100 reactions

Invitrogen™ Vybrant™ DyeCycle™ Ruby Stain, 100 reactions

Vybrant® DyeCycle™ Ruby Stain is a cell permeable DNA dye that can be used for cell cycle analysis on a 488 nm or 635 nm red diode laser.

Features

• Precise, Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant® DyeCycle™ Ruby Stain is an ideal tool for DNA content analysis in living cells since the stain is cell permeable and, after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass.
• Low Cytotoxicity, Unlike stains that require high concentrations or have chemical structures that are toxic to cells, Vybrant® DyeCycle™ Ruby Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
• Minimal Compensation, Well-suited for the popular red 633 nm laser line, the Vybrant® DyeCycle™ Ruby Stain/DNA complex has fluorescence excitation and emission maxima of 638/686 nm, respectively. The spectral characteristics allow use of the red laser for cell cycle analysis and frees up other channels for additional parameters.
• Simple, Robust Staining Protocol, For cell analysis, simply prepare flow cytometry tubes containing 0.5 mL of cells suspended in complete media at a concentration of 5 × 10⁵ cells/mL. To each tube, add 1 µL of Vybrant® DyeCycle™ Ruby Stain and mix well. Final stain concentration is 5 µM. Incubate at 37°C for 15–30 minutes, protected from light. Analyze without washing cells on a flow cytometer using 488 nm or 633/5 nm excitation and >670 nm emission.

Contents

• 1 vial of Vybrant™ DyeCycle™ ruby stain (2.5 mM in DMSO).