Invitrogen™ WT Expression Kit, 30 Reactions

Invitrogen™ WT Expression Kit, 30 Reactions

The WT Expression Kit is an RNA amplification kit that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit.

The first-generation Affymetrix® amplification method requires depletion of ribosomal RNA (rRNA) from RNA samples for optimal exon-level analysis. Conversely, the Ambion® WT Expression Kit uses a novel reverse transcription (RT) priming method that eliminates the need for a separate rRNA depletion step. The kit includes RT primers designed using a proprietary oligonucleotide matching algorithm that eliminates primer sequences with homology to known ribosomal RNAs. The result is complete and unbiased coverage of the transcriptome, with rRNA amplification levels significantly lower than those of other methods.

Features

• Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays
• Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays
• Supports a streamlined workflow that does not require a separate rRNA depletion step
• Is ideal for high-sensitivity expression profiling
• Includes Label or Dye: No
• Labeling Method: Indirect Labeling
• Reverse Transcriptase: Proprietary
• Sample Type: Total RNA
• Starting Material Cell No.: ≥ 50 ng total RNA
• System Type: Affymetrix GeneChip™
• Product Type: WT Expression Kit

Contents

• First-Strand Enzyme Mix: 33 µL 
• First-Strand Buffer Mix: 132 µL 
• Second-Strand Enzyme Mix: 165 µL 
• Second-Strand Buffer Mix: 413 µL 
• IVT Enzyme Mix:• 198 µL
• IVT Buffer Mix: 792 µL
• Control RNA 1 mg/ml: 10 µL
• Nuclease-free Water: 1.75 mL 
•  2nd-Cycle Buffer Mix: 264 µL
• Random Primers: 66 µL
• 2nd-Cycle Enzyme Mix: 264 µL
• RNase H: 66 µL
• Nucleic Acid Binding Buffer Concentrate: 3.3 mL
• Nucleic Acid Binding Beads: 660 µL
• Nucleic Acid Wash Solution Concentrate: 20 mL
• Elution Solution: 5 mL
• Nuclease-free Water: 10 mL
•  8-Strip PCR Tubes & Caps: 10
• U-Bottom Plate: 4 
• Reservoir: 2