Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
100 Reactions
Order number :
20158
Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit
The Thermo Scientific LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.
Features
- Sensitive—chemiluminescent detection comparable to radioactive detection
- Time-saving—develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
- Flexible—compatible with RNA probes biotinylated by different methods
- Easy to use—complete kit includes optimized reagents for binding reactions and RNA probe detection
- Non-radioactive—eliminate waste concerns from radioactive RNA probes
Contents
- 10 µM Unlabeled IRE RNA Control: 25 µL
- 2 mg/mL Cytosolic Liver Extract: 50 µL
- 10 mg/mL tRNA: 100 µL
- 10X REMSA Binding Buffer: 1 mL
- 50% Glycerol: 500 µL
- 2M KCl: 500 µL
- 1M MgCl2: 500 µL
- DTT: lyophilized
- Nuclease-Free Water: 1.5 mL
- 5X REMSA Loading Buffer: 1 mL
- Stabilized Streptavidin-HRP: 1.5 mL
- Luminol/Enhancer Solution: 80 mL
- Stable Peroxide Solution: 80 mL
- Nucleic Acid Detection Blocking Buffer: 500 mL
- 4X Wash Buffer: 500 mL
- Substrate Equilibration Buffer: 500 mL
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