Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

The Thermo Scientific LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

Features

• Sensitive—chemiluminescent detection comparable to radioactive detection
• Time-saving—develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
• Flexible—compatible with RNA probes biotinylated by different methods
• Easy to use—complete kit includes optimized reagents for binding reactions and RNA probe detection
• Non-radioactive—eliminate waste concerns from radioactive RNA probes
  
  

Contents

• 10 µM Unlabeled IRE RNA Control: 25 µL
• 2 mg/mL Cytosolic Liver Extract: 50 µL
• 10 mg/mL tRNA: 100 µL
• 10X REMSA Binding Buffer: 1 mL
• 50% Glycerol: 500 µL
• 2M KCl: 500 µL
• 1M MgCl2: 500 µL
• DTT: lyophilized
• Nuclease-Free Water: 1.5 mL
• 5X REMSA Loading Buffer: 1 mL
• Stabilized Streptavidin-HRP: 1.5 mL
• Luminol/Enhancer Solution: 80 mL
• Stable Peroxide Solution: 80 mL
• Nucleic Acid Detection Blocking Buffer: 500 mL
• 4X Wash Buffer: 500 mL
• Substrate Equilibration Buffer: 500 mL