Thermo Scientific™ Biotin Chromogenic Detection Kit, 10 Reactions
On demandThermo Scientific™ Biotin Chromogenic Detection Kit, 10 Reactions
Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit.
Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment.
Features
- High sensitivity of detection—0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
- Low background due to optimized washing and blocking procedures
- Non-radioactive detection visualization does not require X-ray film or other specific equipment
- Fast and convenient—ready-to-use components
Contents
- Streptavidin-AP Conjugate: 40 μL
- 50X BCIP/NBT Solution: 2 × 2 mL
- Blocking Reagent (powder): 5 g
- 10X Blocking/Washing Buffer: 200 mL
- 10X Detection Buffer: 30 mL
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