Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells, 40 Reactions

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells, 40 Reactions

The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.

Features

• Fast & convenient—5-minute, room-temperature ligation
• Efficient—ccdB gene for positive selection results in >80% clones with correct insert
• Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection

Contents

• Linearized pCR™-Blunt vector
• ExpressLink™ T4 DNA ligase 
• 5X ExpressLink™ T4 DNA ligase buffer
• Control template
• dNTPs
• Sterile water
• M13 forward and reverse primers