Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells, 20 Reactions
On demand
Order number :
K275020
Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells, 20 Reactions
The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.
Features
- Fast & convenient—5-minute, room-temperature ligation
- Efficient—ccdB gene for positive selection results in >80% clones with correct insert
- Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection
Contents
- Linearized pCR™-Blunt vector
- ExpressLink™ T4 DNA ligase
- 5X ExpressLink™ T4 DNA ligase buffer
- Control template
- dNTPs
- Sterile water
- M13 forward and reverse primers
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