Invitrogen™ pcDNA™3.1/Zeo (+) Mammalian Expression Vector
On demandInvitrogen™ pcDNA™3.1/Zeo (+) Mammalian Expression Vector
This pcDNA™3.1/Zeo(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
Cytomegalovirus (CMV) enhancer-promoter for high-level expression
Large multiple cloning site in either forward (+) or reverse (-) orientations
Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Contents
- pcDNA™3.1/Zeo(+): 20 μg
- pcDNA™3.1/Zeo/CAT control: 20 μg
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