Invitrogen™ FISH Tag™ RNA Red Kit, with Alexa Fluor™ 594 dye

Invitrogen™ FISH Tag™ RNA Red Kit, with Alexa Fluor™ 594 dye

FISH Tag™ RNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ RNA Kits in two Alexa Fluor® colors or get the FISH Tag™ RNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts.
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

Features

• Dye (Ex/Em): Alexa Fluor® 594 (590/615 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success

Contents

• Alexa Fluor® reactive dye (Component A): 5 vials in each of 2 pouches
• Dimethylsulfoxide (DMSO) (Component J): 200 μL
• Dithiothreitol (DTT) (Component H): 100 μL
• Glycogen, ultrapure, nuclease free (Component I): 20 μL
• Sodium bicarbonate (Component K): 84 mg
• Water, nuclease free (Component L): 4.0 mL
• RNA nucleotide mix (Component M): 20 μL
• T7 RNA polymerase (Component N): 10 μL
• T3 RNA polymerase (Component O): 10 μL
• SP6 RNA polymerase (Component P): 10 μL
• DNase I (Component Q): 15 μL
• T3/T7 transcription buff  er (Component R): 80 μL
• SP6 transcription buff  er (Component S): 40 μL
• RNaseOUT™ ribonuclease inhibitor (recombinant) (Component T): 10 μL
• SlowFade® Gold antifade reagent (Component G): 2 mL
• Binding buff  er (Component B): 6.0 mL
• Wash buff  er (Component C): 3.2 mL
• Elution Buff  er (Component D): 6.0 mL
• Spin columns and collection tubes (Component E): 20 columns with tubes
• Collection tubes (Component F): 20 tubes