Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays

Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays

The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip® workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript® II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

Features

• Labeling Method: Direct Labeling
• Remarks: For generating double-stranded cDNA
• System Type: Affymetrix GeneChip™
• Sample Type: Poly(A+) RNA, Total RNA
• Labeling Target: cDNA
• Detection Method: Biotin-Based
  
  

Contents 

• T7-Oligo(dT), 50 μM: 60 μL
• 5X 1st-Strand Reaction Mix: 120 μL
• DTT, 0.1 M: 60 μL
• dNTP Mix, 10 mM (10 mM each dATP, dCTP, dGTP, dTTP): 120 μL
• SuperScript® II RT (200 U/μl): 60 μL
• 5X 2nd-Strand Reaction Mix: 900 μL 
• E. coli DNA Ligase (10 U/μl): 30 μL 
• E. coli DNA Polymerase I (10 U/μl): 120 μL
• RNase H (2 U/μl): 30 μL 
• T4 DNA Polymerase (5 U/μl): 60 μL
• RNase-free water: 3.1 mL
• EDTA, 0.5 M: 300 μL