Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL), 1000

Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL), 1000

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity

Features

• Thermostability—optimal activity at 37°C
• Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR

Contents

• 1 × 1 ml M-MLV RT (200 U/µl)
• 1 ml 5X First Strand Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM Magnesium Chloride]
• 500 µl 100 mM DTT