Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL), 200
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
200 Reactions
Order number :
28025013
Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL), 200
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity
Features
- Thermostability—optimal activity at 37°C
- Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7 kb
- Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
Contents
- 1 × 200 µl M-MLV RT (200 U/µl) 1 ml
- 5X First Strand Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM Magnesium Chloride]
- 500 µl 100 mM DTT
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