Invitrogen™ pAO815 Pichia Expression Vector

Invitrogen™ pAO815 Pichia Expression Vector

pAO815 is a Pichia expression vector designed to clone multiple copies of a gene into a single vector. The vector contains a Bgl II site upstream of the 5´ AOX1 gene and a unique BamH I site downstream of the 3´ AOX1 transcription termination (TT) signal.

Four steps are required to generate multiple copies of the gene of interest:

• The gene is cloned into the unique EcoR I site in the vector.
• The construct is digested with BamH I and Bgl II to release the "expression cassette" containing the AOX1 promoter, gene of interest, and 3´ AOX1 TT.
• Concatemers of the expression cassette are generated by ligation in vitro.
• The multiple copies are inserted back into the pAO815 vector and transformed into Pichia. This vector is included in the Multi-Copy Pichia Expression Kit.

Contents

• pAO815: 20 µg