Invitrogen™ pFLD1 Pichi pastoris Expression Vector Kit

Invitrogen™ pFLD1 Pichi pastoris Expression Vector Kit

pFLD and pFLD-alpha are vectors used to express recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to a C-terminal peptide containing the V5 epitope and a polyhistidine (6xHis) tag.

The vector allows high-level, methanol- and methylamine-inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain, including X-33, GS115, SMD1168H, and KM71H.

 The pFLD vectors contain the following elements:

• 5' fragment containing the FLD1 promoter for methanol- or methylamineinduced expression of the gene of interest
• Zeocin™ resistance gene for selection in both E. coli and Pichia
• Ampicillin resistance gene for selection in E. coli
• C-terminal peptide containing the V5 epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion protein (if desired)

Contents

Contains the following vectors, supplied at a concentration of 0.5 µg/µL in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a total volume of 40 µL:

• pFLD
• pFLD-alpha
• pFLD/CAT (control plasmid)