Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit, 20 Reactions

Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit, 20 Reactions

The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression.

In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.

Features

• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation

Contents

• pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/µl): 20 µL 
• dNTP Mix: 10 µL
• Salt Solution: 50 µL 
• Sterile Water: 1 mL 
• T7 Sequencing Primer (0.1 µg/µl): 20 µL
• BGH Reverse Sequencing Primer (0.1 µg/µl): 20 µL 
• Control PCR Template (0.1 µg/µl): 10 µL 
• Control PCR Primers (0.1 µg/µl each): 10 µL 
• Expression Plasmid (0.5 µg/µl): 10 µL 
• S.O.C. Medium: 6 mL
• TOP10 Cells: 21 × 50 µL
• pUC19 Control DNA: 50 µL