Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit, 20 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 Kit
Order number :
K490001
Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit, 20 Reactions
The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression.
In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
Features
- A proofreading enzyme is used for fewer errors in cloned genes
- Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
Contents
- pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/µl): 20 µL
- dNTP Mix: 10 µL
- Salt Solution: 50 µL
- Sterile Water: 1 mL
- T7 Sequencing Primer (0.1 µg/µl): 20 µL
- BGH Reverse Sequencing Primer (0.1 µg/µl): 20 µL
- Control PCR Template (0.1 µg/µl): 10 µL
- Control PCR Primers (0.1 µg/µl each): 10 µL
- Expression Plasmid (0.5 µg/µl): 10 µL
- S.O.C. Medium: 6 mL
- TOP10 Cells: 21 × 50 µL
- pUC19 Control DNA: 50 µL
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