Invitrogen™ PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit, 20 Preps

Invitrogen™ PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit, 20 Preps

The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 1.5 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
NOTE: The use of a vacuum manifold (A35899) is required to perform this procedure. We recommend our FastVac Vacuum Manifold.

Features

• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
• High yields—isolate up to 1.5 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
  

Contents

• Resuspension buffer[1] (red): 2 x 150 mL 4°C
• Lysis buffer[2] (blue): 2 x 150 mL
• Precipitation buffer (yellow): 2 x 150 mL
• Binding buffer: 2 x 150 mL
• Wash buffer 1: 2 x 55 mL
• Wash buffer 2 (concentrate): 2 x 23 mL
• Elution buffer: 12 mL
• Column assembly[3]: 20 columns
• Syringe filters: 20 filters
• Syringe plungers: 20 plungers
• Collection tubes: 20 tubes
• Quick reference card: 1 card