Invitrogen™ Quant-iT™ microRNA Assay Kit

Invitrogen™ Quant-iT™ microRNA Assay Kit

The Quant-iT™ microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.

Features

• Accurately detects as little as 0.5 ng miRNA in a microplate well
• Core dynamic range of 5–500 ng/mL
• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL
• For Use With (Equipment): Fluorometer, Microplate Reader
• Sample Type: RNA (<40 bases), Tissue (FFPE), Total RNA, miRNA, mRNA (Bacterial), Total RNA (Yeast), Total RNA (Bacterial), mRNA
• Assay: microRNA Quantitation
• Excitation/Emission: 498/518
• For Use With (Equipment): Fluorometer, Microplate Reader
• No. of Reactions: 1000 (200 μL Assay Volume)
• Quantitation Range: 1 to 100 ng
• Detection Method: Fluorescent
  
  

Contents

• Quant-iT™ microRNA reagent (Component A): 1.0 mL 
• Quant-iT™ microRNA buffer (Component B): 250 mL
• microRNA standards (21-mer) (Component C): set of 8, 500 μL each