Invitrogen™ QuantiGene™ Plex 384-well Assay Kit

Invitrogen™ QuantiGene™ Plex 384-well Assay Kit

The QuantiGene Plex 384-well Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 384-well plates and must be used with a target-specific QuantiGene Plex panel (sold separately). 
The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets. The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals, which are read with a Luminex xMAP instrument (FlexMap3D).

Features

• Accomodates difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
• True multiplexing—measures up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity
• Standardized platform—uses 384-well plate format compatible with Luminex FlexMap3D systems
• Simple workflow—employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling
• Large inventory of validated genes—select from over 22,000 genes to create pathway and disease-themed panels
• Rapid turnaround for custom panels—a custom panel can be created within three weeks
• For Use With (Equipment): Invitrogen™Luminex™ FLEXMAP 3 D™ Instrument System
  
  

Contents

• Proteinase K: 200 µL
• Blocking Reagent: 2.1 mL
• Label Probe Solution: 31 mL
• Pre-Amplifier Solution: 31 mL
• Amplifier Solution: 31 mL
• SAPE: 280 µL
• SAPE Diluent: 31 mL
• Lysis Mixture: 24 mL
• Wash Buffer Component 1: 6 mL
• Wash Buffer Component 2: 100 mL
• SAPE Wash Buffer: 1000 mL
• Hybridization plates (Day 1) 384-well: 2
• Manual Pressure seals (Day 1): 4
• Heat seals (Day 1): 4
• Magnetic Separation plates (Day 2): 2
• Plates seals (Day 2): 10