Invitrogen™ Qubit™ RNA HS Assay Kit, 500
On demandInvitrogen™ Qubit™ RNA HS Assay Kit, 500
The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer.
For use with equipment: Qubit™ Fluorometer.
Features
- Core range (high confidence): 25 ng/mL to 500 ng/mL
- Extended ranges (moderate confidence): 20 ng/mL to 25 ng/mL and 500 ng/mL to 1000 ng/mL
- Useful for quantitation of samples for microarray, RT-PCR, and northern blot procedures
- Accurate in the presence of DNA, salts, solvents, proteins, and free nucleotides
- Excitation/Emission: 644/673
- For Use With (Equipment): Qubit Fluorometer
- Quantitation Range: 20 to 1000 ng
- Shipping Condition: Room Temperature
- Detection Method: Fluorescent
Contents
- Qubit® RNA HS Reagent (Component A) , 1.25 mL
- Qubit® RNA HS Buffer (Component B), 250 mL
- Qubit® RNA HS Standard #1 (Component C), 5 mL
- Qubit® RNA HS Standard #2 (Component D), 10 × 500 μL
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