Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

The Thermo Scientific Pierce RNA 3'-End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.
The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex. The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit.


Features

• Non-radioactive – a single biotin labels has similar detection sensitivity as radioactivity
• Easy to use – RNA ligase and optimized reaction buffer are included
• Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes
• End-labeled – results in minimal disturbance of RNA secondary structure
• Flexible – desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin
  
  

Contents

• Desthiobiotinylated Cytidine Bisphosphate: 40 µL
• T4 RNA Ligase: 40 µL
• T4 RNA Ligase Reaction buffer (10X): 100 µL
• Non-labeled RNA Control: 100 µL
• Biotinylated IRE RNA Control: 35 µL
• RNase Inhibitor: 2 x 10 µL
• DMSO: 200 µL
• PEG (30%): 300 µL
• Glycogen: 20 µL
• Nuclease-free Water: 1.5 mL