Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit

Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit

The RNAqueous®-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (~100 cells to ~1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.

Features

• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations

Contents

• DNase I Buffer: 10X600 µL
• DNase I 2 U/µl: 45 µL
• Formaldehyde Load Dye: 1 mL
• Linear Acrylamide 5 mg/ml: 100 µL
• Positive Control Primers: 50 µL
• 5M Ammonium Acetate:1 mL
• Water for 64% Ethanol: 35 mL
• DNase Inactivation Reagent: 600 µL
• Lysis/Binding Solution: 30 mL
• Wash Solution 1: 25 mL
• Wash Solution 2/3: 35 mL
• Pestles for tissue disruption: 3
• Filter Cartridges: 30
• Collection Tubes: 60
• Elution Solution: 25 mL