Invitrogen™ RNAqueous™-96 Automated Kit

Invitrogen™ RNAqueous™-96 Automated Kit

The Ambion® RNAqueous®-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous®-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.

Features

• Format: 96-well plate
• High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
• Starting Material Amount: Up to 1.0 mg tissue, Up to 5 x 10^5 cells
• For Use With (Equipment): MultiPROBE™ II HT, Biomek™ 2000
  
  

Contents

• Filter Plates: 4
• Collection Plates with lids: 4
• Culture Plates: 4
• Heat Plate with lid: 1
• Nuclease-free Water: 100 mL
• Elution Solution: 100 mL
• Lysis/Binding Solution: 100 mL
• Rebinding Mix: 100 mL
• Wash Solution Concentrate: 2 x 175 mL
• DNase 1 (20 U/μL): 1.25 mL
• DNase 1 Buffer: 12 mL