Invitrogen™ RNAqueous™-Midi Total RNA Isolation Kit

Invitrogen™ RNAqueous™-Midi Total RNA Isolation Kit

The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.

Features

• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding
• to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not
• required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Contents

• Elution Solution: 100 mL
• Collection Tubes: 30
• Syringe Filter Units: 15
• Water for 64% ethanol : 36 mL
• Wash Solution #1: 130 mL
• Wash Solution #2/3 Concentrate: 252 mL
• Lysis/Binding Solution: 100 mL
• Lithium Chloride Precipitation Solution: 25 mL
• Formaldehyde Load Dye: 6 mL