Invitrogen™ RNAqueous™-Micro Total RNA Isolation Kit

Invitrogen™ RNAqueous™-Micro Total RNA Isolation Kit

The RNAqueous®-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.

Features

• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples

Contents 

• Micro Filter Cartridge Assembly: 50 
• Micro Elution Tubes: 50 
• Wash Solution 1 Concentrate: 15 mL  
• Wash Solution 2/3 concentrate: 28 mL 
• Lysis Solution: 25 mL
• LCM Additive: 200 µL
• DNase 2 U/µL: 55 µL
• 10X DNase I Buffer: 600 µL
• DNase Inactivation Reagent: 150 µL
• Elution Solution: 5 mL