Invitrogen™ Second Strand cDNA Synthesis Kit, 200

Invitrogen™ Second Strand cDNA Synthesis Kit, 200

Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features

• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents

Contents

• Second Strand Enzyme Mix, 4 x 1 mL
• 5X Second Strand Reaction Mix, 4 x 250 µL
• 0.5 M EDTA, pH 8.0, 4 x 1 mL
• RNase I, 10 U/µL, 4 x 500 µL
• Water, nuclease-free, 12 x 1.25 mL