MGIEasy Exome Capture V5 Probe Set（16 RXN）

MGIEasy Exome Capture V5 Probe Set（16 RXN）

MGIEasy Exome Capture V5 Probe Set not only covers the regions of traditional exome probes, but also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc. The primary targets with a design size of 69Mb include: CCDS, GENCODE, RefSeq, miRbase and MGI Clinical Database. As a universal exome capture probe set, it is compatible with both of MGI and other NGS platforms.

Features

• Probe regions 69Mb, The databases used for probe design include CCDS, GENCODE, RefSeq, miRBase and so on.
• More comprehensive gene coverage, 455 genes can be 100% covered.
• Superior coverage and uniformity, When the mean sequencing depth is 100X, 96% of the regions can reach coverage of 20X.
• Stable and efficient capture rate, The capture rate is over >60% (by reads).
• Probe lenght: 90nt.
•  Covered regions, Traditional exome regions and various diseases related regions.
•  DNA input, 1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridization and 1μg ≤total input≤2μg 
• Number of samples per hybridization: 1-8.
• Technologies: Capture+NGS.

Content

• Probes, MGIEasy Exome Capture V5 Probe（16RXN): 1000007741
• Hybridization and elution, MGIEasy Exome Capture Elution Kit（16RXN): 1000007742

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