Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli, 50 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
500 Reactions
Order number :
K465040
Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli, 50 Reactions
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. They use a pCR™-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants.
Features
- 3´-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR™2.1-TOPO®) or T7 and SP6 (pCR™II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
Contents
Box 1:
- Topoisomerase I-activated pCR™II-TOPO™ vector
- PCR buffer
- Salt solution
- dNTPs
- Control template
- M13 forward and reverse primers
- Control PCR primers
- Sterile water
Box 2:
- One Shot™ Chemically Competent or Electrocomp™ E. coli
- S.O.C. medium
- Supercoiled pUC19 control plasmid.
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/2w1szqZhmi0fuIQfe2eLT05Oz1hvheAma5XQS75s.jpg)
Invitrogen™ Champion™ pET160 Gateway™ Expression Kit with Lumio™ Technology
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/3f5d7cd4-eef8-45e7-b1d8-834063383431.jpg)
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli, 20 Reactions
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/3c2104e2-1d8c-408f-adf5-e578027ccece.jpg)
Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells, 20 Reactions
(Ex-work price)
$ On demand
$ On demand