Invitrogen™ Ulysis™ Alexa Fluor™ 594 Nucleic Acid Labeling Kit
On demandInvitrogen™ Ulysis™ Alexa Fluor™ 594 Nucleic Acid Labeling Kit
ULYSIS® Nucleic Acid Labeling Kits provide a unique method to attach a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast—just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.
We developed this series of ULYSIS® kits to enable rapid and simple coupling of our Alexa Fluor® dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS™), is based on the use of a platinum dye complex (owned by KREATECH Diagnostics) that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.
The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS™ complex can be accomplished through the use of a simple spin-column procedure. DNA longer than ∼1,000 base pairs requires a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.
Features
- Dye (Ex/Em): Alexa Fluor® 594 (588/615 nm)
- Labeling reaction is complete in as little as 15 minutes
- Available in several Alexa Fluor® dye colors
Contents
- ULS® labeling reagent (Component A):1 vial for 20 labelings or 5 vials for 4 labelings each (U21650)
- Dimethylformamide (DMF, 50% solution in water) or Dimethylsulfoxide (DMSO) (Component B): 200 μL
- Labeling buffer (Component C): 600 μL
- Deoxyribonuclease I (DNase I) (Component D): 100 μg
- DNase I storage buffer (Component E): 200 μL
- 10X DNase I reaction buffer (Component F): 500 μL
- DNA from calf thymus (Component G): 100 μL
- Nuclease-free H2O (Component H): 5 mL
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