Ion Torrent™ Ion AmpliSeq™ Mouse BCR IGH SR Assay, RNA

Ion Torrent™ Ion AmpliSeq™ Mouse BCR IGH SR Assay, RNA

The Ion AmpliSeq Mouse BCR IGH SR Assay, RNA, is a robust, targeted next-generation sequencing (NGS) assay for use in basic and translational immunology, immuno-oncology, hemato–oncology, and vaccine research. It is designed to accurately identify and measure the clonal expansion of B lymphocytes in blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen (FF) and formalin-fixed paraffin-embedded (FFPE) samples. The assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor (BCR) immunoglobulin heavy (IGH) chain from genomic DNA template . The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone tracking and measurements of B-cell clonal expansion and diversity. Analysis of IGH CDR3-region amino acid motifs may reveal signatures of B-cell responses to defined antigens.

Features

• Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
• High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
• Efficient workflow with 48 hour sample-to-results time Flexible input requirements ranging from 100 ng to 1 µg
• Streamlined and user-friendly informatics solution with with multi-sample analysis functionality

Contents

• Ion AmpliSeq BCR IGH-SR Assay, RNA
• Ion AmpliSeq Library Kit Plus
• Ion NGS RT Kit