Ion Torrent™ Ion Plus Fragment Library Kit

Ion Torrent™ Ion Plus Fragment Library Kit

As an integral component of the Personal Genome Machine™ sequencing platform, the Ion Plus Fragment Library Kit is designed to produce high quality DNA libraries via multiple possible workflows. The economically priced Ion Plus Fragment Library Kit provides low cost sample preparation, bringing next generation sequencing to every lab. This revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries when combined with either physical shearing methods or our proprietary Ion Shear™ enzymatic fragmentation technology (Ion Xpress Plus Library Kit) , thus saving time and cost. With significantly higher yields than other library construction techniques, high quality libraries can be attained with as little as 100 ng input DNA. In addition, this highly efficient library construction process enables the creation of “amplification-free" libraries from <1 ug input material. This kit is also extremely flexible in that it is compatible with multiple physical shearing and library size selection methods.

The Ion Plus Fragment Library Kit contains sample preparation reagents for library construction of up to 20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. Store components at -20°C.

For use with equipment: Ion PGM™ System, Ion OneTouch™ System.

Features

• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes with a choice of mix-and-match workflows
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

Contents

• 5× End Repair Buffer,1 tube, 400 µL
• End Repair Enzyme,1 tube, 20 µL
• 10× Ligase Buffer,1 tube, 200 µL
• DNA Ligase, 1 tube, 40 µL
• Adapters, 1 tube, 100 µL
• Platinum™ PCR SuperMix High Fidelity, 2 tubes, 1 mL
• Library Amplification Primer Mix,1 tube
• Nick Repair Polymerase,1 tube, 160 µL
• dNTP Mix, 1 tube, 40 µL
• Low TE, 2 tubes, 1.5 mL