Ion Torrent™ Oncomine™ TCR Pan-Clonality Assay (TRB, TRG, FR3-J), DNA

Ion Torrent™ Oncomine™ TCR Pan-Clonality Assay (TRB, TRG, FR3-J), DNA

The Oncomine TCR Pan-Clonality Assay is a targeted next-generation sequencing (NGS) assay for detection of T-cell diversity to measure clonal expansion/contraction with accuracy and for detection of rare clones with high sensitivity. Primers have been specifically designed to sequence the FR3-J regions of the TCR beta chain and TCR gamma chain. Through the use of Ion AmpliSeq technology, sequencing of the CDR3 region of the beta and gamma T-cell receptor chains occurs in a single reaction. When combined with Ion Reporter Software, data can be easily analyzed and reports created within a short period of time.

For use with equipment: GeneStudio.

Features

• Sequencing of CDR3 region of both TRB and TRG chains
• Reduced secondary testing—Ion AmpliSeq technology enables single reaction to sequence beta and gamma chains
• Detect rare clones at LOD of 10-6
• Optimized Oncomine informatics pipeline

Contents

• Oncomine TCR Pan-Clonality Panel
• dNTP mix
• Ion AmpliSeq Library Plus Kit