Ion Torrent™ Oncomine™ BCR IGHV Leader-J Assay, DNA

Ion Torrent™ Oncomine™ BCR IGHV Leader-J Assay, DNA

The Oncomine BCR IGHV Leader-J Assay is a targeted next-generation sequencing (NGS) assay for detection of somatic hypermutation (SHM) in the variable region of the immunoglobulin heavy chain of B-cell receptors. SHM increases the affinity of the B-cell receptor for antigen, post-VDJ recombination, and is used as a biomarker in chronic lymphocytic leukemia (CLL). Primers have been specifically designed to target the leader and joining regions of the immunoglobulin heavy chain, resulting in an amplicon ∼480 bp in length. When combined with Ion Reporter Software, you can easily analyze your data and create reports within a short period of time.

For use with equipment: GeneStudio.

Features

• Sequencing of variable region of IGH; primer from Leader and Joining regions
• Assay meets current ERIC guidelines for research in CLL
• Coverage of entire IGH variable gene for accurate measurement of somatic hypermutation rate
• Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots

Contents

• Oncomine IGHV Leader-J Panel
• dNTP mix
• Ion AmpliSeq Library Plus Kit