Ion Torrent™ Oncomine™ Myeloid Research Assay, 96

Ion Torrent™ Oncomine™ Myeloid Research Assay, 96

The Ion Torrent Oncomine Myeloid Research Assay consists of three pools of AmpliSeq oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent platforms. The assay is designed to provide sensitive and comprehensive sample amplification of relevant DNA mutations and fusion transcripts associated with myeloid disorders in a single NGS run. The panel is comprised of 40 key DNA genes and a broad fusion panel of 29 driver genes to cover the most relevant targets in major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).

Features

• Simple, robust workflow that encompasses mutations and fusions in two DNA and one RNA pool in a single NGS run
• Excellent coverage of challenging targets such as CEBPA and FLT3-ITDs
• Multiplex up to four samples on a single Ion 318 chip or twelve on an Ion 530 chip
• Compatible with as little as 10 ng/pool input DNA or RNA per library from blood or bone marrow samples
• Validated detection of somatic variants down to 5% allele frequency
• Simple and fast workflow produces targeted libraries in 3.5 hours typically
• For use on the Ion PGM and Ion S5 systems with manual or automated library preparation
• Compatible with the Oncomine Knowledgebase Reporter for variant annotation

Contents

• 5X Oncomine™ Myeloid DNA Panel (pool 1 of 2)
• 5X Oncomine™ Myeloid DNA Panel (pool 1 of 2)
• 5X Oncomine™ Myeloid RNA Panel (pool 1 of 1)
• 5X Ion AmpliSeq™ HiFi Mix (red cap)
• FuPa Reagent (brown cap)
• Switch Solution (yellow cap)
• DNA Ligase (blue cap)
• 25X Library Amp Primers[1] (white cap)
• 1X Library Amp Mix (black cap)
• Low TE (clear cap)