The Newest in Laser Technology for Clinical Applications
- 300 mW laser power and one microsecond pulse length.
- Laser and RED-i® target locator built into 40x objective.
- Multi-pulse mode for fast and easy trophectoderm biopsy.
- High image quality.
- Improved working distance - use with or without glass heater.
- No laser alignment needed.
- Similar in size to standard objectives.
- Compatible with all major microscope models.
Laser and RED-i® Target Locator built into 40x Objective
- 40x, 0.60 N.A., I.R.
- High transmission in the UV [>350 nm], visible and near-infrared, long working distance
- Infinity corrected for 1 mm thick polystyrene Petri Dish, on 500 μm glass heating plate. A nose cap corrector is available if use without the glass heating plate is required.
- Working Distance: 1.7 mm to glass heater plate
- Parfocality: < 40 µm
- Compatibility: 3 RMS thread adapters permit installation on all major microscope models
The RED-i speeds workflow by allowing you to position the cell under the laser beam without looking at the monitor:
- Built into the LYKOS laser objective.
- Red LED indicator spot visible through microscope eyepieces.
- Adjustable brightness level.
- Always remains in focus.
- Laser beam is NOT transmitted through eyepieces - SAFE for your eyes.
- Simple alignment process.
Introducing the Multi-Pulse Feature for the LYKOS Laser System
The Multi-Pulse software gives our lasers an automated, precise tool that enables rapid trophectoderm biopsy.
The Multi-Pulse feature provides rapid, repeated firing of the laser to facilitate removal of cells from an embryo during the trophectoderm biopsy process. Trophectoderm biopsy is considered one of the best methods used to remove cells from the embryos of patients undergoing pre-implantation genetic diagnosis (PGD) to screen for genetic disease or aneuploidy.
Prior to release of the Multi-pulse software, individual laser shots were required to weaken or break the junctions between the trophectoderm cells so that they can be aspirated into the biopsy micropipette. The significant advantage of Multi-Pulse is that one press of the remote footswitch initiates multiple laser pulses in rapid succession for fast and easy cell separation, thereby limiting the amount of time the embryo spends outside the incubator.
Professor Barry Behr, Co-Director, REI/IVF Program, Department of Obstetrics and Gynecology at the Stanford University School of Medicine, a long-time user of Hamilton Thorne lasers and a pioneer in trophectoderm biopsy, commented, “The Multi-Pulse capability, which provides the user with the ability to automatically fire the laser multiple times at relatively low power, will contribute significantly to the efficiency and safety of trophectoderm biopsy.”
Installs on Any Inverted Microscope
Since the LYKOS laser is based on the standard objective height, it easily installs on turrets of all models of inverted microscopes, even older models. Also, the unique design of the laser allows unrestricted access to other installed objectives. And you can switch the LYKOS laser from one brand of microscope to another - without need of physical laser realignment or customization.
Hands-free Laser Firing
When working with micromanipulators, the LYKOS allows you to work in "hands-free" mode by using the optional foot switch to fire the laser. Simply use the manipulators to position the embryo to the target and press the foot switch to fire the laser. Your micromanipulation proceeds rapidly and the time the embryo spends on the microscope stage is minimized.
Free Your Microscope Ports
The HT lasers leave both the fluorescent light port and the filter cube port open for normal use (OOsight® compatible). Other laser systems use one of these ports to install their laser, which can interfere with full microscope functionality. The HT lasers are the only laser systems which don't require removal of the laser to use fluorescence.
No Physical Laser Alignment by End-User is Needed
The ZILOS-tk and LYKOS lasers are physically aligned under strictly controlled conditions at the factory and locked in place, so you do not have to perform tedious alignment procedures on-site. Simply position the computer generated target to the laser spot and you are ready to go!
Simply attach the laser-objective to the microscope turret and you are ready to go! Each laser comes with a variety of turret adapters to allow installation on all major models of inverted microscopes.
Digital Color Camera - Seamless Video Transfer to Monitor!
The digital camera available with both the desktop and laptop laser system offers a seamless stream of video from camera to monitor:
- No jerky motion or image delay.
- No need to resize image window to reduce delay.
- Video refresh rate not slowed down by graphic overlay.
For a complete laser system, including full featured software and computer, the portability of the laser laptop configuration can't be beat. All laser components fit into a custom designed carry case, which offers both protection and easy transport. Combine this with a laptop unit and a small digital camera, and you can literally walk out the door with a "ZILOS-tk or LYKOS To Go!"
Choice of Computer
Based on your need for bench space and portability, the LYKOS or ZILOS-tk may be configured with either a desktop or laptop computer.
PATENTED Isotherm Rings™ - The Original Embryo Safeguard
Isotherm Rings™ (Patent Issued July 4, 2006) help the user prevent potential harmful effects on blastomeres adjacent to the zona due to heat from the laser drilling.
The Isotherm Rings™ appear on the screen as a series of six concentric circles of varying colors (left) and indicate the maximum temperature reached at the ring diameter at various laser pulse durations (right). At longer pulse duration, it is apparent that temperatures radiate farther into the center of the embryo, increasing the likelihood of blastomere damage. The orange ring (second from center) is also a useful indicator of the drill hole size at the selected pulse duration.
With this interactive Isotherm Rings™ feature, a user can now "see" the heating and drill hole size and eliminate the temperature "guesswork."
TheLYKOS and ZILOS-tk provide the highest level of flexibility, compatibility, and ease of use. Features include:
- Real-time video recording
- Auto-image capture on laser fire
- Thumbnail image capture
- Measure saved images
- Measurements are displayed with value labels
- 2x, 4x, 8x zoom and user-defined image panning
- Calibrate and store multiple objectives
- Option to save overlays with images
- One-click save all images
- Choose which levels of Isotherm Rings™ to display
Choose between a circle, arrow, or the unique Isotherm Rings as your target locator. The Isotherm Rings appear on the screen as a series of six concentric circles of varying colors, and indicate the drill hole size and the maximum temperature reached at the ring diameter. The Isotherm Rings automatically adjust for every power and pulse time combination. In addition, you may select which rings to view.
The Documentation option provides the ability to measure captured images using the built-in measurement tools. In addition, the software automatically calculates the mean and standard deviation. The measured and calculated values are transferred into the report at the touch of a button. You may also choose to create a report that can hold 2 images and measurement data, or 4 images with no measurement data. Images may also be saved with the overlay of measurements made. Data also may be saved into an ASCII format.More
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