Stiletto® laser

Stiletto® laser

The Stiletto research laser system combines a proprietary infrared laser objective, controller software and an automated stage for various procedures applicable to adherent cells in culture. The laser module attaches to the turret of your inverted microscope to easily target the cell monolayer to ablate, isolate or score into uniform sections.

Benefits of Stiletto

• Streamline workflow with Stiletto’s automated features
• Select multiple areas across an entire plate and Stiletto automatically ablates cells inside or outside of each designated zone, or along a path
• Integrates seamlessly with your current workflow: no extra preparation needed for your cell samples
• Quickly scores cell monolayer into sections of user-defined dimensions
• Offers precision technology that provides consistent results between different users
• Reduces risk of contamination: non-contact laser means plate lid is kept in place during laser application
• Cell characteristics and gene expression maintained throughout laser-assisted sequential passaging
• Flexible solution applicable to a variety of research needs

Features

• 20x objective module with integrated laser attaches to the turret like a standard objective
• Automated X-Y-Z stage with “remembered focus” at each selected area
• Proprietary software interface features include:
• Modes for auto-scoring, ablation within selected area, outside selected area and along a perimeter
• User-specified well map to easily navigate from well to well
• Designate multiple target areas and modes throughout the entire plate/dish
• Stores parameters and automatically returns to each location to perform the user-defined tasks
• Ability to select target areas under a lower magnification, thereby providing a larger field of view
• Uses your standard petri dishes and multi-well plates - no need to purchase “special” dishes

Stiletto Laser Module & Controller:

STILETTO Laser Module: 20x Objective and Class 1, infrared wavelength, 300 mW Laser combined into a single compact turret mounted unit. The Stiletto laser module installs on all current and older models of inverted microscopes. The LWD 20x objective is specially designed and manufactured according to high quality HT specifications. It is constructed to have high transmission in the infrared, as well as in the visible.

Laser Controller: High speed micro controller with instantaneous closed loop laser power feedback control.

Accessories Included

• Motorized Stage with Plate Holder: X-Y motorized stage: [Prior Scientific, ProScan] Customized for make and model specific inverted microscope
• Microtitre plate holder for motorized stage: [Prior H223RNC] 85x128mm, recessed, for well plates with chamfered corners. Compatible with most 6-, 24-, 96- and 384-well plates.
• Color Digital Camera and C-Mount Adapter: Digital (USB 2.0) color camera (max resolution 1392 x 1040). All Stiletto systems also come with the appropriate c-mount adapter for your microscope.
• Turret Adapters and Hex Key: A set of four turret adapters is included with each Stiletto. These adapters allow you install the Stiletto on any inverted microscope.
• High Performance Computer System: Desktop computer including digital image capture board, all required cables, DVD-RW, mouse and keyboard. A 20 inch, flat screen monitor provides clear visualization of embryo and saves space on the workbench.