MGIEasy Exome Universal Library Prep Set (16 RXN)

MGIEasy Exome Universal Library Prep Set (16 RXN) 

MGIEasy Exome Universal Library Prep Set aims to prepare libraries with human gDNA. DNA is fragmented by Covaris, followed by the steps of adaptor ligation at both ends and PCR amplification. The amplified PCR product can be used for exome capture with MGIEasy Exome Capture V4/V5 Probe Set or other commercialized probes.

Features

• Compatible with exome probes from various companies, The constructed libraries are compatible with probes from Agilent, Nimblegen, IDT,and etc.
• Compatible with the automation systems, Multiple steps can be finished with the automation systems MGISP-100, such as steps from fragmented DNA to pre-PCR, as well as post-capture elution to post-PCR. 
• Quick to prepare libraries and easy to operate, Library preparation time 7h (without hybridization capture time), hands-on time ~30min.
• Required sample input, 10 ng -1000 ng fragmented gDNA; DNA concentration: ≥12.5 ng/μL; intact or mildly degraded DNA; D260/280=1.8~2.0.
• Compatible probes, Probes MGI, Agilent, Nimblegen, IDT.
•  Library fragment size, 200-600 bp.

Content

• Core library preparation kit, MGIEasy Universal DNA Library Prep Module: 1000019376 
• Adaptors, MGIEasy DNA Adapters-16 (Tube) Module: 1000019378
• Clean beads, MGIEasy DNA Clean Beads: 1000005278
• Circularization, MGIEasy Circularization Kit V2.0: 1000005260
• Other, MGIEasy Exome Capture Accessory Kit: 1000007743

Related Products

• MGIEasy Exome Capture V4 Probe Set（16 RXN): 1000007745
• MGIEasy Exome Capture V5 Probe Set（16 RXN): 1000007746