Applied Biosystems™ GeneChip™ WT Terminal Labeling Kit, 30 Reactions

Applied Biosystems™ GeneChip™ WT Terminal Labeling Kit, 30 Reactions

The Whole Transcript (WT) Sense Target Labeling Assay has an updated protocol. The GeneChip WT (Whole Transcript) Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip WT Sense Target (ST) Labeling Assay.
The WT Terminal Labeling Kit employs a strategy for reproducible DNA fragmentation with a combination of uracil DNA glycosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE 1). The typical input material is random-primed, single-stranded DNA
Following fragmentation, the resulting fragmented DNA can then be labeled using terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound, GeneChip DNA Labeling Reagent. Both components are also provided in the WT Terminal Labeling Kit. The final labeled DNA target is ready to be added to the cocktail for hybridizing to the arrays such as the GeneChip Human Exon 1.0 ST Array.

Contents

• 10X cDNA Fragmentation Buffer
• UDG, 10U/μL
• APE 1, 1000U/μL
• TdT, 30U/μL
• 5X TdT Buffer
• DNA Labeling Reagent, 5mM
• RNase-free Water