Invitrogen™ BioPrime™ Total FFPE Genomic Labeling System

Invitrogen™ BioPrime™ Total FFPE Genomic Labeling System

The BioPrime® Total FFPE Genomic Labeling System is a complete genomic DNA labeling kit designed for use in array comparative genomic hybridization applications (aCGH) using formalin-fixed paraffin-embedded (FFPE) samples. Previous protocols have required large amounts of very high quality DNA, which is often difficult to obtain from FFPE material. With a random prime amplification (RPA) method, using labeling mixes specifically formulated for FFPE samples, BioPrime® Total FFPE Genomic Labeling System generates high yields of labeled DNA which is most representative of the starting sample.. Increased call accuracy is achieved with higher yields of labeled material on the array, giving you more accurate calls.

Features

• Improved call rate with higher signal to noise ratios from Alexa Fluor® 3 and 5 dyes
• Easy cleanup with PureLink™ purification eliminates the need for complicated volume-reduction steps
• Less channel bias due to enhanced reaction formulation for FFPE samples
• Lower requirements for input material using enzymatic RPA method
• Excitation/Emission: 650/670, 555/565
• Includes Label or Dye: Yes
• Labeling Method: Direct Labeling
• No. of Reactions: 30
• Product Type: Genomic Labeling System
• Detection Method: Fluorescent
• Final Product Type: Probes (Labeled DNA)
• Labeling Target: Genomic DNA
• Label or Dye: Alexa Fluor™ 3, Alexa Fluor™ 5
• Sample Type: Tissue (FFPE), FFPE tissue
  

Contents

• Alexa Fluor® 3 FFPE 10X Nucleotide Mix: 3 × 25 μL 
• Alexa Fluor® 5 FFPE 10X Nucleotide Mix: 3 × 25 μL
• Exo– Klenow Fragment, 40 U/μl: 3 × 30 μL 
• Stop Buffer: 0.5 mL 
• 2.5X Random Primers Solution: 3 × 0.25 mL
• Control DNA (Salmon Sperm) (10 mg/ml): 10 μL 
• DEPC-treated water: 2 × 0.5 ml