Thermo Scientific™ Active Rap1 Pull-Down and Detection Kit

Thermo Scientific™ Active Rap1 Pull-Down and Detection Kit

The Thermo Scientific Pierce Active Rap1 Pull-Down and Detection Kit is a complete kit for selective enrichment and detection of GTP-bound Rap1 GTPase through specific protein interaction with the RalGDS protein-binding domain.

The Active Rap1 Pull-Down and Detection Kit includes purified GST-RalGDS Rap-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/wash buffer, anti-Rap1 antibody, sample buffer, spin columns and collection tubes. The kit was validated using lysates from NIH 3T3 cells, a cell line that is known to have robust Rap1 activity.

Features

• Highly sensitive and accurate—optimized reagents, specific anti-Rap1 antibody and Western blot procedure ensure accurate controls and semi-quantitative results
• Validated—functionally tested for Rap1 detection to ensure quality and performance
• Compatible—effective with a variety of cell types from mouse, rat and human sources

Contents

• GST-RalGDS-RBD Fusion Protein, 1 vial 
• Anti-Rap1 Antibody, 5 units, 50 µL 
• Glutathione Agarose Resin, 3 mL 
• GDP (100X), 50 µL 
• GTPyS (100X), 50 µL 
• SDS Sample Buffer (2X), 1.5 mL 
• Lysis/Binding/Wash Buffer (1X), 100 mL 
• Spin Cups, 30 cups 
• Collection Tubes, 90 tubes