Thermo Scientific™ Active Rap1 Pull-Down and Detection Kit
On demand
Order number :
16120
Thermo Scientific™ Active Rap1 Pull-Down and Detection Kit
The Thermo Scientific Pierce Active Rap1 Pull-Down and Detection Kit is a complete kit for selective enrichment and detection of GTP-bound Rap1 GTPase through specific protein interaction with the RalGDS protein-binding domain.
The Active Rap1 Pull-Down and Detection Kit includes purified GST-RalGDS Rap-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/wash buffer, anti-Rap1 antibody, sample buffer, spin columns and collection tubes. The kit was validated using lysates from NIH 3T3 cells, a cell line that is known to have robust Rap1 activity.
Features
- Highly sensitive and accurate—optimized reagents, specific anti-Rap1 antibody and Western blot procedure ensure accurate controls and semi-quantitative results
- Validated—functionally tested for Rap1 detection to ensure quality and performance
- Compatible—effective with a variety of cell types from mouse, rat and human sources
Contents
- GST-RalGDS-RBD Fusion Protein, 1 vial
- Anti-Rap1 Antibody, 5 units, 50 µL
- Glutathione Agarose Resin, 3 mL
- GDP (100X), 50 µL
- GTPyS (100X), 50 µL
- SDS Sample Buffer (2X), 1.5 mL
- Lysis/Binding/Wash Buffer (1X), 100 mL
- Spin Cups, 30 cups
- Collection Tubes, 90 tubes
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