Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe

Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe

Thermo Scientific Serine Hydrolase Probes are ActivX™ Fluorophosphonate (FP) and other tagged phosphonate probes to purify or assay serine hydrolase active sites using fluorescence, biotin-affinity, or mass spectrometry.

These ActivX™ FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enrichment, TAMRA fluorophore for detection or a reactive- azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins

Features

• Specific—labels the reactive site of active serine hydrolases
• Compatible—tags available for capture, detection and Staudinger conjugation
• Flexible—use for in vitro or intracellular enzyme labeling