Thermo Scientific™ LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)
On demand
Order number :
21651
Thermo Scientific™ LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)
Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Features
- Reactive groups: NHS ester and pyridyldithiol
- Reactive towards: amino and sulfhydryl groups
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Water-insoluble (dissolve first in DMF or DMSO)
- SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cells
- Compare to other varieties of SPDP-type reagents, including pegylated forms
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