Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL, 1 Column

Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL, 1 Column

The AminoLink Plus Immobilization Kit contains AminoLink Plus Coupling Resin and additional reagents needed to perform 5 protein immobilizations, with expected yields of 2 to 40 mg protein per column. The AminoLink Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Features

• AminoLink Plus CouplingResin—aldehyde-activated crosslinked 4% beaded agarose
• Ideal for antibodies and other proteins— immobilize molecules via primary amines (-NH2)
• Flexible coupling conditions—efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (citrate-carbonate, pH 10) coupling protocols provided
• Stable, permanent immobilization—Coupling reaction esults in stable, leak-resistant secondary amine bond between resin and ligand
• Better than immobilization to CNBr-activated agarose—bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
• Versatile and reusable—prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

Contents

• AminoLink Plus Columns: 2 mL, 1 columns
• PBS Pack (makes 500mL): 1 pack
• Citrate-Carbonate Buffer Pack (makes 500mL): 1 pack
• Sodium Cyanoborohydride Solution, 5M: 0.5 mL
• Quenching Buffer: 15 mL
• Wash Solution: 50 mL
• Column Accessories